猪嗜血支原体猪体感染试验

doi:10.11843/j.issn.0366-6964.2013.10.019

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (10): 1645-1653.doi: 10.11843/j.issn.0366-6964.2013.10.019

猪嗜血支原体猪体感染试验

王帅彪，李玉峰^*，常晨，程王琨，马培培，邹垚

（南京农业大学动物疫病诊断与免疫重点开放实验室，南京 210095）

收稿日期:2013-04-23 出版日期:2013-10-23 发布日期:2013-10-23
通讯作者: 李玉峰，副教授，E-mail:yufengli@njau.edu.cn; Tel:025-84395504
作者简介:王帅彪(1988- )，男，河南许昌人，硕士生，主要从事动物传染病预防控制研究，E-mail: 2010107063@njau.edu.cn
基金资助:
公益性行业（农业）科研专项项目（200903036-10）

Study on Infection of Mycoplasma suis to Pigs

WANG Shuai-biao, LI Yu-feng^*, CHANG Chen, CHENG Wang-kun, MA Pei-pei, ZOU Yao

(Key Laboratory of Animal Diseases Diagnostic and Immunology of Ministry of Agriculture,College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China)

Received:2013-04-23 Online:2013-10-23 Published:2013-10-23

摘要/Abstract

摘要：

为研究猪嗜血支原体致病机制，将其感染健康猪和切脾猪，从临床症状、血液学和生物化学指标、菌血症、抗体水平以及细胞因子、病理剖检和组织学检查等方面系统研究猪嗜血支原体病的发病过程。结果显示，与不切脾猪相比，切脾猪体内的猪嗜血支原体含量（血液中含量1.0×10⁸拷贝·mL^－1）更高，维持时间更长。猪嗜血支原体血液中含量和白细胞计数、血细胞比容、红细胞计数和铁等指标显著相关。仅1头猪产生猪嗜血支原体抗体，3组猪血清中均没有检测到细胞因子IL-4 和IFN-γ。本研究复制出猪嗜血支原体病病例，记录了该病的发展过程，为该病的病理机制、预防、诊断等方面的研究奠定基础。

Abstract:

Porcine eperythrozoonosis (PE) caused by Mycoplasma suis (M. suis) infection is a febrile acute icteroanemia in pigs and human. In order to study the pathomechanism of PE, clinical symptoms, hematological parameters, M. suis loads, cellular and humoral immunity, gross pathology and histopathology were system recorded to monitor the process of this disease in models of splenectomized and non-splenectomized pigs. The result showed that the M. suis loads in splenectomized pigs maintain higher level (1.0×10⁸ copies per mL) and longer time than that of non-splenectomized pigs. Correlations between M. suis loads and the values of WBC, hematocrit, RBC and Fe were significant. The antibody against MSG1 was detected in only one pig, whereas the IL-4 and IFN-γ were not detected in all of the groups during the whole experiment. PE was reproduced and the process of this disease was recorded in this study which plays a fundamental role in revealing the pathomechanism, providing the guideline of prevention and diagnosis of this disease.

中图分类号:

S852.62

王帅彪，李玉峰，常晨，程王琨，马培培，邹垚. 猪嗜血支原体猪体感染试验[J]. 畜牧兽医学报, 2013, 44(10): 1645-1653.

WANG Shuai-biao, LI Yu-feng, CHANG Chen, CHENG Wang-kun, MA Pei-pei, ZOU Yao. Study on Infection of Mycoplasma suis to Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1645-1653.

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参考文献

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猪嗜血支原体猪体感染试验

Study on Infection of Mycoplasma suis to Pigs

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